|
KMID : 0861020110260010065
|
|
Korea Journal of Herbology
2011 Volume.26 No. 1 p.65 ~ p.74
|
|
|
Molecular biologic mechanism of obesity by GGEx18
|
|
Lee Hee-Young
Yoon Ki-Hyeon
Seo Bu-Il
Park Gyu-Ryel
Yoon Mi-Jung
Shen Zhi-Bin
Cui Hong-Hua
Shin Soon-Shik
|
|
|
Abstract
|
|
|
Objectives: This study was undertaken to verify the modulation mechanism of Gyeongshingangjeehwan18 (GGEx18) in ob/ob male mice.
Methods: Eight-week old mice (wild-type C57BL/6J and ob/ob) were used for all experiments. Wild-type C57BL/6J mice were used as lean control and obese ob/ob mice were randomly divided into 5 groups : obese control, GGEx15 (Ephedra sinica Stapf + Rheum palmatum L.), GGEx16 (Ephedra sinica Stapf + Laminaria japonica Aresch), GGEx17 (Rheum palmatum L. + Laminaria japonica Aresch), and GGEx18 (Ephedra sinica Stapf + Laminaria japonica Aresch + Rheum palmatum L.). After mice were treated with several kinds of GGEx for 11 weeks, the mRNA expression of peroxisome proliferator-activated receptor (PPAR) target genes and uncoupling protein (UCP) were measured. In addition, PPARα and PPARβ transactivation was examined in NMu2Li hepatocytes, C2C12 myocytes, and 3T3-L1 preadipocytes using transient transfection assays.
Results:1. Hepatic PPARα target genes, such as ACOX and VLCAD mRNA levels were significantly increased by GGEx18 compared with obese controls. In skeletal muscle, LCAD mRNA expression was stimulated by GGEx16, GGEx17, and GGEx18, whereas MCAD mRNA expression by GGEx17 and GGEx18. PPARβ target LPL mRNA levels were also increased by GGEx16, GGEx17, and GGEx18 in skeletal muscle, but adipose LPL mRNA levels were decreased. In addition, GGEx18 upregulated UCP mRNA expression in skeletal muslce. 2. PPARα reporter gene expression was increased by GGEx18 in NMu2Li cells compared with vehicle. PPAR and PPARβ reporter activities were also increased by all GGEx treatments in C2C12 and 3T3-L1 cells.
Conclusions :These results suggest that GGEx can act as PPARα and PPARβ activators, and that GGEx may regulate obesity by stimulating PPARα, PPARβ, and UCP activity. Of the 4 compositions, GGEx18 seems to be most effective in improving obesity and lipid disorders.
|
|
|
KEYWORD
|
|
|
Ob/Ob mouse, Gyeongshingangjeehwan18 (GGEx18), PPARα, PPARβ, obesity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|